TNF alpha Kit ELISA

N° du produit ABIN6210920

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Poulet TNF alpha.

Aperçu rapide pour TNF alpha Kit ELISA (ABIN6210920)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Poulet

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit TNF alpha Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Chicken Tumor Necrosis Factor (TNF) ELISA Kit

Analytical Method: Quantitative

Sensibilité: 18.8 pg/mL

Ingrédients: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Matériel non inclus:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Information d'application

Indications d'application: Optimal dilutions/concentrations should be determined by the end user.

Commentaires:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plaque: Pre-coated

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Tumor Necrosis Factor Alpha (TNFA) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Tumor Necrosis Factor Alpha (TNFA) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Date de péremption: 6 months

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor

Sujet: F1DFK9_CHICK

UniProt: F1DFK9

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome